10 Some recent examples of its use in different applications include: protein folding/unfolding, 11 thermal stability profiles, 12 comparisons of wild type and mutant proteins, 13 monitoring continuous flow dynamics measurements, 14 identifying similarities in protein evolutionary relationships, 15 comparisons of new spectra with downloaded Protein Circular Dichroism Data Bank (PCDDB) components, 16 demonstrations of fidelity of folding of expressed proteins, 17 instrumentation comparisons and calibrations, 18 as well as “on-the-fly” displays, data processing and analyses at SRCD beamlines. The only requirement for the user is that they cite this paper if they use the software for data processing and/or analyses.ĬDtool has been widely used as a processing and analysis tool in CD and SRCD studies on diverse samples, including proteins, 4 nucleic acids 5 and even chiral small molecules, 6 as well as for examining samples in different physical forms such as monolayers, 7 fibers, 8 emulsions, 9 and oriented samples. This version along with sample test data, and instructions for installation, is freely downloadable without registration. CDtoolX includes a wide range of functions for displaying, processing, analyzing, and archiving CD and SRCD data. It also runs on Mac (OS X) and Linux platforms that have Windows 10 emulators installed. Hence, we have developed CDtoolX, a downloadable software package with most of the previous functions retained or upgraded and a number of additional ones added it has been primarily designed for use on Windows 10 (hence the designation “X” in the software name) machines, but is also usable on Windows 7 (and below) platforms. However, large changes associated with the developments of Windows 8 and then Windows 10 operating systems rendered it generally unusable, whilst the user community was still keen on having access to a version for these different platforms. This is quite a long lifetime for a downloadable software package. It was developed for Windows systems, and throughout many upgrades of the Windows operating systems (and Windows emulators, which have enabled use on other operating systems such as MACs), it has remained stable and usable. 3ĬDtool software has been freely available to registered users. gen format and so nearly all have this as their main or optional output/input format. It has been utilized by more than 700 registered users over the past 14 years.įortunately, unlike commercial CD instruments, the usage of different formats has not been as common on SRCD instruments, since most of these were developed after the introduction of the CDtool. In 2004, we created a downloadable software package (CDtool) 2 for processing and analysis of CD spectroscopic data with inputs in a range of different formats and units, and which would output results in a common “.gen” format. Lab-based CD instruments from different manufacturers all produce their own types of output in different (and sometimes proprietary) formats, which make comparisons of data sets produced on different instruments challenging and time consuming. Both lab-based CD instruments and instruments which use synchrotron radiation as their light sources (SRCDs) 1 produce information on optical features (mostly in the ultraviolet or vacuum ultraviolet wavelength ranges) which can provide conformational information. Circular dichroism (CD) spectroscopy is a highly used method for biophysical characterizations of a wide range of biological molecules, including proteins, nucleic acids, peptides, and sugars.
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